What Centrifugation Is and Why Its Used

What Centrifugation Is and Why Its Used The term centrifuge can refer to a machine that houses a rapidly rotating container to separate its contents by density (noun) or to the act of using the machine (verb). The modern device traces its origins to a spinning arm apparatus designed in the 18th century by engineer Benjamin Robins to determine drag. In 1864, Antonin Prandtl applied the technique to separate milk and cream. His brother refined the technique, inventing a butterfat extraction machine in 1875. While centrifuges are still used to separate milk components, their use has expanded to many other areas of science and medicine. Centrifuges are most often used to separate different liquids and solid particulates from liquids, but they may be used for gases. They are also used for other purposes than mechanical separation. How a Centrifuge Works A centrifuge gets its name from centrifugal force the virtual force that pulls spinning objects outward. Centripetal force is the real physical force at work, pulling spinning objects inward. Spinning a bucket of water is a good example of the forces at work. If the bucket spins fast enough, the water is pulled into it and doesnt spill. If the bucket is filled with a mixture of sand and water, spinning it produces centrifugation. According to the sedimentation principle, both the water and sand in the bucket will be drawn to the outer edge of the bucket, but the dense sand particles will settle to the bottom, while the lighter water molecules will be displaced toward the center. The centripetal acceleration essentially simulates higher gravity, however, its important to keep in mind the artificial gravity is a range of values, depending on how close an object is to the axis of rotation, not a constant value. The effect is greater the further out an object gets because it travels a greater distance for each rotation. Types and Uses of Centrifuges The  types of centrifuges are all based on the same technique but differ in their applications. The main differences between them are the speed of rotation and the rotor design. The rotor is the rotating unit in the device. Fixed-angle rotors hold samples at a constant angle, swinging head rotors have a hinge that allows sample vessels to swing outward as the rate of spin increases, and continuous tubular centrifuges have one chamber rather than individual sample chambers. Very high-speed centrifuges and ultracentrifuges spin at such a high rate that they can be used to separate molecules of different masses or even isotopes of atoms. For example, a gas centrifuge may be used to enrich uranium, as the heavier isotope is pulled outward more than the lighter one. Isotope separation is used for scientific research and to make nuclear fuel and nuclear weapons. Laboratory centrifuges also spin at high rates. They may be large enough to stand on a floor or small enough to rest on a counter.  A typical device has a  rotor with angled drilled holes to hold sample tubes. Because the sample tubes are fixed at an angle and centrifugal force acts in the horizontal plane, particles move a tiny distance before hitting the wall of the tube, allowing dense material to slide down. While many lab centrifuges have fixed-angle rotors, swinging-bucket rotors are also common.  These machines are used to isolate components of  immiscible liquids  and  suspensions. Uses include separating blood components, isolating DNA, and purifying chemical samples. Medium-size centrifuges are common in daily life, mainly to quickly separate liquids from solids. Washing machines use centrifugation during the spin cycle to separate water from laundry, for example. A similar device spins the water out of swimsuits. Large centrifuges may be used to simulate high-gravity. The machines are the size of a room or building. Human centrifuges are used to train test pilots and conduct gravity-related scientific research. Centrifuges may also be used as amusement park rides. While human centrifuges are designed to go up to 10 or 12 gravities, large diameter non-human machines can expose specimens to up to 20 times normal gravity.  The same principle may one day be used to simulate gravity in space.   Industrial centrifuges are used to separate components of colloids (like cream and butter from milk), in chemical preparation, cleaning solids from drilling fluid, drying materials, and water treatment to remove sludge. Some industrial centrifuges rely on sedimentation for separation, while others separate matter using a screen or filter. Industrial centrifuges are used to cast metals and prepare chemicals. The differential gravity affects the phase composition and other properties of the materials. Related Techniques While centrifugation is the best option for simulating high gravity, there are other techniques that may be used to separate materials. These include filtration, sieving, distillation, decantation, and chromatography. The best technique for an application depends on the properties of a sample and its volume.

Example Sentences of the Verb Know

Example Sentences of the Verb Know This page provides example sentences of the verb Know in all tenses including active and passive forms, as well as conditional and modal forms. Base Form know / Past Simple knew / Past Participle known / Gerund knowing Present Simple He knows a lot of people in Paris. Present Simple Passive The President is known to be in trouble. Present Continuous None Present Continuous Passive None Present Perfect They have known each other for years. Present Perfect Passive The facts in the case have been known since last year. Present Perfect Continuous None Past Simple She knew it was time to leave. Past Simple Passive The story was known by all in the room. Past Continuous None Past Continuous Passive None Past Perfect They had known about the problem before they told him. Past Perfect Passive The problem had been known about by all before they told him. Past Perfect Continuous None Future (will) She will know its you. Future (will) passive You will be known by everybody in the room. Future (going to) She is going to know the answer soon. Future (going to) passive The answer is going to be known at the end of the lesson. Future Continuous None Future Perfect They will have known Jack for twenty years by the end of this month. Future Possibility She might know the answer. Real Conditional If she knows the answer, she will tell you. Unreal Conditional If she knew the answer, she would tell you. Past Unreal Conditional If she had known the answer, she would have told you. Present Modal Andy should know the answer. Past Modal Andy should have known the answer. Quiz: Conjugate with Know Use the verb to know to conjugate the following sentences. Quiz answers are below. In some cases, more than one answer may be correct. They _____ about the problem before they told him.The President _____ to be in trouble.The facts in the case _____ since last year.They _____ Jack for twenty years by the end of this month.If she _____ the answer, she will tell you.The story _____ by all in the room at the trial yesterday.They _____ each other for years.He _____ a lot of people in Paris.If she _____ the answer, she would have told you.She _____ its you. Quiz Answers had knownis knownhave been knownwill have knownknowswas knownhave knownknowshad knownwill know

Commonwealth bank of Australia Essay Example | Topics and Well Written Essays - 1250 words

Commonwealth bank of Australia - Essay Example It is approximately one century since this giant financial institution was formed by the Australian government. However it is currently owned by private investors owing to the move by the Australian government to privatize the bank in 1996. Commonwealth Bank of Australia has continued to expand its operations in various regions through acquisitions, joint partnership and takeover. Commonwealth Bank steady performance in the industry is attributed to a wide range of strategies that include customer satisfaction, business banking, technology and operational excellence, profitable growth as well as trust and team spirit1. From the financial point of view it is evident that Commonwealth Bank has emerged strongly from a humble beginning to be a profitable banking institution in Australia. This clam can be justified by the fact that the Group’s net earnings after tax for the financial year ended 2011/12 hit $6,394 million which is 13% increase compared to the previous year. The Grou p recorded 18.4% and 411.2 cents on Return on equity and Earnings per share respectively. This was a 12% increase compared to prior financial year. The Group declared a final dividend of $ 1.88 per share which was an 11% increase compared to previous financial year2. ... re 10198 Home loans 335841 Construction 3199 Personal 17968 Asset financing 9584 Other commercial and industrial 106762 RATIO’s OF BANK EXAMPLE: RISK RATIO ANALYSIS FOR COMMONWEALTH BANK OF AUSTRALIA BANK OVER THE PERIOD 2010 TO 2010 Ratio Ratio formula Actual figures for Year †¦Ã¢â‚¬ ¦(previous year) ($M) Ratio answer for Year†¦Ã¢â‚¬ ¦ (previous year) Actual figures for Year †¦Ã¢â‚¬ ¦( current year) ($M) Ratio answer for Year†¦Ã¢â‚¬ ¦ (current year) Source of information in annual report Comments Interest margin Interest revenue – interest expenses Average earning assets 32,215 – 20,293 553,735 0.02 37,304 – 24,697 576,369 0.02 Income statement p98 and note 2 page 119. The interest margin remain staeble over the two financial period owing to effective risk management strategies Net margin (after Tax) Operating profit after tax Interest income + non-interest income 5,680 32, 215+ 7,366 0.14 6,410 37,304 + 6,790 0.15 Income statement p98 and note 2 p119 The increase in net margin is as a result of Loans, bills discounted and other receivables Asset utilization Interest income + non-interest income Assets 32, 215+ 7,366 646,330 0.06 37,304 + 6,790 667,899 0.07 Income statement p98 and balance sheet p100 The increase in asset utilization ration indicates that the groups management effectively utilized the groups assets . Return on assets Operating profit after tax Equity 5,680 35,570 0.16 6,410 37,287 0.17 Income statement p98 and balance sheet p100 The increase in the return on assets ratio is attributable to the significant increase in the groups long-term assets Leverage multiplier Assets Equity 646,330 35,570 18 667,899 37,287 17 Balance sheet p100 The reduction in the ratio indicates that the group has reduced the level of debt used to finance its capital structure

Lorex Pharmaceuticals Essay Example | Topics and Well Written Essays - 1250 words

Lorex Pharmaceuticals - Essay Example This essay discusses that Lorex Pharmaceuticals has developed and holds all patent rights to Linatol, a new high blood pressure medicine. Since the pharmaceutical industry operates in a monopolistically competitive atmosphere (where no specific pharmaceutical company dominates the market), the patent-holder of a new product can have considerable market advantage over other companies. Lorex pharmaceutical is therefore seeking solutions to optimize this competitive advantage by balancing cost, productivity, and quality with regards to the production of Linatol. To reach this end, the managers of Lorex Pharmaceuticals must determine and select a target amount to which each of the 10-ounce bottles of the product would be filled. This task takes into consideration two issues: 1) Specifying higher fill targets will lead to higher material cost but fewer seconds, and 2) use of the one-standard-deviation rule can cause production delays. Using tests to determine the optimal fill-line, it has been established that 10.17-ounce mark would result in optimal production and maximum returns. Since probability analysis is only suggestive and the accuracy increases with samples size, it is recommended that more tests be conducted to reach more accurate results. Quality assurance managers of Lorex Pharmaceuticals must determine and select a target amount to which each of the 10 ounce bottles of Linatol, a new high blood pressure medicine, would be filled. ... The pharmaceutical industry is ever changing, as can be seen from the many changes in the structure of markets and organizations that occur over the years. Competition in the industry is intense, which means that mergers, acquisitions, etc. are not uncommon. In the pharmaceutical industry in the U.S., there is an extreme contrast between a specific products market during patent enforcement (where a patent holder may control over 80 percent of the product's market and can charge premium prices) and after the patent expires (where market control is reduced to about 30 percent because of the many sellers and buyers that soon emerge after patent expiration). Lorex Pharmaceuticals has developed Linatol, a new high blood pressure medicine, and the company holds all patents right to it. A company that develops a new drug has the potential to realize very large profits. Decisions in manufacturing budgets and product specifications influence the capacity of a particular product line. These, in turn influence the productivity and actual profit of the company. Thus, manufacturers must take into consideration the cost and quality factors that go into production. It is not unusual for companies to pursue trade-offs in cost, productivity, and efficiency in order to pursue specific markets. This however, can result in poor product quality. This case analysis aims to present some solutions that would help Lorex Pharmaceuticals strike a balance between cost, productivity, and quality. Possible Decision Alternatives Quality assurance managers of Lorex Pharmaceuticals must determine and select a target amount to which each of the 10 ounce

Jackson Pollock and Modern Art Essay Example | Topics and Well Written Essays - 1000 words

Jackson Pollock and Modern Art - Essay Example The essay "Jackson Pollock and Modern Art" explores the art of Jackson Pollock and his impact in the context of Modern Art. He was â€Å"a roughshod, ill-mannered, prodigiously ambitious, aggressive, alcoholic, tormented artist .† This image is important to an interpretation of the apparently chaotic nature of much of Pollock’s works, and the technique that led to them. However, as is often the case with creative artists of all sorts, and particularly painters such as Pollock, there was a method to his madness. As Toynton has pointed out, films of Pollock creating his paintings clearly show that even the most abstract of them start as figurative works and only move into the abstract as they develop.Pollock moved his canvas from the easel to the floor, thus enabling him to work on much larger canvases with greater ease than before, and also to see them from multiple points of view. In one revealing statement he talked about his technique and why he used it: My painting d oes not come from the easel. I hardly ever stretch the canvas before painting. I prefer to tack the unstretched canvas to the hard wall or the floor. I need the resistance of a hard surface. On the floor I am more at ease. I feel nearer, more part of the painting, since this way I can walk around it, work from the four sides and literally be in the painting. It is interesting to note that Pollock takes an intense and yet casual approach to his painting. Thus the fact that he does not go through the often laborious process. of stretching the canvas before painting on it, but rather merely tacking it to a wall or floor illustrates the casual, almost primitive method of preparation. At the same time Pollock becomes more intensely involved with the painting, as if he were actually a part of it: I continue to get further away from the usual painter's tools such as easel, palette, brushes, etc. I prefer sticks, trowels, knives and dripping fluid paint or a heavy impasto with sand, broken glass or other foreign matter added. When I am in my painting, I'm not aware of what I'm doing. It is only after a sort of 'get acquainted' period that I see what I have been about. I have no fear of making changes, destroying the image, etc., because the painting has a life of its own. I try to let it come through. It is only when I lose contact with the painting that the result is a mess. Otherwise there is pure harmony, an easy give and take, and the painting comes out well. (Varnedoe, 23) The idea of being "in" the painting is of course hardly new to Pollock, but the fact that his technique apparently fitted into his emotional and intellectual attachment to the painting is. His physical technique: standing on and thus within the painting, had a profound effect both upon his creations and upon generations of creative artists in general and painters in particular, for years both during and after his lifetime. Pollock hinted, although never explicitly stated, that he was influenced by Native American sand paintings, which are made by trickling thin lines of colored sand onto a flat surface. After WWII, in 1947 Pollock began what he called his "action paintings", which were at least partially informed by the surrealist ideas of "psychic automatism". This automatism was meant to be a direct expression of the unconscious. A direct expression of something which is, by definition, unknowable to the conscious mind might seem a

Factors Affecting Healing Capabilities of the Body

Factors Affecting Healing Capabilities of the Body Analyse Health Information James Rowe 1.3) Intracellular fluid makes up approximately 60-67% of total body fluid. With Intracellular fluid making up approximately 33-40% in both these compartments it is water making up a majority of the solvent in the different body compartments. The total body weight that the fluid makes up is about 60% in Men and about 55% in Women. This is lower due to the higher fat content. Fluid Physiology: 2.1 Fluid Compartments. 2014. Fluid Physiology: 2.1 Fluid Compartments. [ONLINE] Available at: http://www.anaesthesiamcq.com/FluidBook/fl2_1.php. [Accessed 13 February 2014]. An electrolyte is an electrically charged ion that can affect the amount of water in the body. It is also responsible for determining the acidity of the blood (alters PH). And is responsible for a change in muscle function. Sodium maintains the osmotic pressure and volume of the extracellular fluid within the body. Whereas the intracellular fluid is controlled by potassium. By effecting these the body can effect the amount of fluid being drawn into the ECF and ICF. 2.1) Dehydration is a danger because the body requires water to maintain homeostasis. Water is also a key ingredient in blood volume. The less water in the blood the thicker it becomes. The function of the skin have been impaired because the skin integrity itself is compromised. Depending on wound depth many problems can occur. 1st degree burns à ¯Ã†â€™Ã‚   Superficial skin 2nd degree burns à ¯Ã†â€™Ã‚   Some underlying skin damage 3rd degree burns à ¯Ã†â€™Ã‚   All skin layers damage After an area of the body has been burnt many things will happen. Including an increase in the amount of WBC that migrate to the area to help fight off infection and start repairing the body. Also the capillaries of the damaged area will be blocked off to prevent and blood loss. And to stop a possible entrance route for any blood infection. Factors affecting the healing capabilities of the body can be categorised into 2 category’s Internal and External. Internal à ¯Ã†â€™Ã‚   Health status (any other illness that may prevent healing) Age Lifestyle Nutritional status External à ¯Ã†â€™Ã‚   Mechanical damage Stress Temperature Debridement of a wound can happen through many different methods. Including surgically, chemically or even by the body’ defence itself. Debridement is where unhealthy tissue is removed from the wound to aid healing. The top 2 layers of the skin are used in a full thickness graft. These are the Dermus and EpiDermus. 3.1) OBS chart Neurovascular OBS Fluid balance chart Referral/Appointment 4.2) Hypertension is high blood pressure. This is where the blood in the artery is being forced around the body under a higher pressure than normal. Arteriosclerosis is the thickening and hardening of the artery walls. Meaning that there is a loss in the elasticity of the artery wall. Hypertension and Arteriosclerosis are often related. Due to the pressure hypertension puts on the arterial wall they lose their elasticity and become hard and thick (Arteriosclerosis) Hypertension is referred to as ‘the silent killer’ due to the fact so many people are unaware that they have the disease. The signs and symptoms of hypertension are very discreet and can go un-diagnosed for a lifetime. Cardiovascular disease and old age are often associated. This is because the development of the disease over time and the disease becoming worse with age. Some changes that can be to reduce the risk of developing the disease are improving diet, don’t live under stress for prolonged periods of time, tests for and hereditary factors. If there is an increased amount of platelets in the blood and therefore you have a very high clotting factor then you may be at risk of a blockage in a blood vessel. 5.2) A blue or purple colouration of the skin is referred to as cyanosis. Hypoxia is where an area of the body is deprived of adequate oxygen supply. Hypoxia can be generalized or local. Ischemia is the insufficient blood flow to a tissue Hypoxemic hypoxia is where the arterial content of O ² insufficient. This can be caused by a pulmonary embolus or a sudden change in the environment pressure (Diving) 5.3) A tracheostomy is a surgical incision into the next. Opening the airway straight through a hole in the trachea. Endotracheal is a type of tracheal tube inserted through the mouth or nose. Both are a type of tracheal tube that’s purpose is to act as a catheter to establish and maintain a airway. And to ensure enough oxygen gets into the body to allow adequate gas exchange. 5.4) Anaemia is a decrease in the number of RBC or less than normal amount of haemoglobin. This will cause the patient to complain of SOB due to lack of oxygen being supplied to the body due to the lack of haemoglobin. 5.5) The problems that Paul is experiencing with breathing can be due to the muscle spasm down the airway. And the swelling narrows the airway makes it difficult for Paul to breathe. An increase in the production of mucus also explains the wheeze in Pauls breathing. This is because the air being exhaled is being forced through mucus. 6.1) If left untreated then the appendix will rupture causing toxicity in the body. In some extreme cases this poisoning of the body can cause death. 6.2) Because without fibre in the diet stools become difficult to pass. Causing a higher pressure and stronger muscle contraction to be needed. Over a prolonged period this causes damage to the intestine. 6.3) Bile is required to breakdown and aid digestion. If the bile duct is blocked then this would hinder the digestion of some protein and fats. The is because of the bile not reaching the areas that it is meant to. This would mean the body would not be able to absorb essential nutrients during the digestive process meaning there would be a deficiency in the body. 6.4) Jaundice (Yellow skin) Pale colour stool. Dark Urine 8.2) BP increase amount of oxygen to muscles. Vasodilation aids this. Heart beat increase rate of blood pumps around the body and increases the amount of gas exchange occurring in the lungs to help the muscle fuel. Resp Rate increases to allow as much oxygen as possible into the body and to get rid of the CO2. This all allows muscles adequate energy for a fight or flight response. 9.1) When blood enters the kidney (Site of urine formation) it passes through juxtaglomerular that detect blood pressure and blood volume before entering the nephron. When in the Nephron blood needs to be under pressure to force out fluid and solutes (smaller than protein) After reabsorption occurs the remaining waste is urine. Without a suitable BP or BV this wouldn’t occur. 10.1) Concave lenses. This is because the positive diopetes of myopic eye. This will help her see things further away (Refrence Marieb) 10.2) Sensorineural deafness – Degeneration or damage to the nerve, neuron and receptor cell. The MP3 caused damage to the nerves in the ear and the neurons of the auditory cortex. This is more serious than conductive deafness as it effects the ability to hear both conduction routes (Air conduction and Bone conduction) Conduction deafness – Something interferes with sound vibration waves. This means that waves do not reach the inner ear fluid (Ear wax and Blockage)

Waters Representation of Freedom in Twains The Adventures of Huckleberry Finn :: essays research papers

In the novel, The Adventures of Huckleberry Finn, the Mississippi River serves as a prominent setting. Huck, a rapscallion who runs away from his dad by faking his death, and Jim, a runaway slave who previously knew Huck, meet up on Jackson’s Island via the river. To Jim, the river is a symbol of freedom and a way to learn. To Huck, the river is a symbol of his life and everything he wants. The open waters bring about bonding, fun times, and a safe house for both characters. Amidst the water brings a deeper meaning of the river than just water, it is a great entity that shows freedom. Jim, the runaway slave, uses this river to escape from Miss Watson, his owner, because she planned on selling him for $800. The river flows to the free states, and this is where Jim plans on going with or without Huck. As he is with Huck more and more, Jim learns emotions, like love and compassion, on the river. Jim’s newly shown emotions come into play when the narrator states, â€Å"It was Jim’s voice – nothing ever sounded so good before. I run along the bank a piece and got aboard, and Jim he grabbed me and hugged me, he was so glad to see me† (Twain 112). Love and compassion is shown here by Jim, to Huck. Only freedom has given this runaway slave a way to learn and live like a human being should. Huck, the rapscallion who fakes his death, also has a deep relationship with the river. He relates the river to his life more or less. Since he is a free man, freedom wouldn’t be the right word, more blithe you could say. â€Å"It was kind of lazy and jolly, laying off comfortable all day, smoking and fishing, and no books nor study† (24). Huck’s nature of a care-free person is shown here. He does not like to be cramped up or reformed to anybody he is not. â€Å"[I] said there warn’t no home like a raft, after all. Other places do seem so cramped up and smothery, but a raft don’t. You feel mighty free and easy and comfortable on a raft† (113). Huck likes to be free and easy like the flowing river. It is undeniable that a slow-flowing river and Huck’s life are inter-related. To both of the characters, who meet up via this entity of water, the river symbolizes a place to bond and safety. Water's Representation of Freedom in Twain's The Adventures of Huckleberry Finn :: essays research papers In the novel, The Adventures of Huckleberry Finn, the Mississippi River serves as a prominent setting. Huck, a rapscallion who runs away from his dad by faking his death, and Jim, a runaway slave who previously knew Huck, meet up on Jackson’s Island via the river. To Jim, the river is a symbol of freedom and a way to learn. To Huck, the river is a symbol of his life and everything he wants. The open waters bring about bonding, fun times, and a safe house for both characters. Amidst the water brings a deeper meaning of the river than just water, it is a great entity that shows freedom. Jim, the runaway slave, uses this river to escape from Miss Watson, his owner, because she planned on selling him for $800. The river flows to the free states, and this is where Jim plans on going with or without Huck. As he is with Huck more and more, Jim learns emotions, like love and compassion, on the river. Jim’s newly shown emotions come into play when the narrator states, â€Å"It was Jim’s voice – nothing ever sounded so good before. I run along the bank a piece and got aboard, and Jim he grabbed me and hugged me, he was so glad to see me† (Twain 112). Love and compassion is shown here by Jim, to Huck. Only freedom has given this runaway slave a way to learn and live like a human being should. Huck, the rapscallion who fakes his death, also has a deep relationship with the river. He relates the river to his life more or less. Since he is a free man, freedom wouldn’t be the right word, more blithe you could say. â€Å"It was kind of lazy and jolly, laying off comfortable all day, smoking and fishing, and no books nor study† (24). Huck’s nature of a care-free person is shown here. He does not like to be cramped up or reformed to anybody he is not. â€Å"[I] said there warn’t no home like a raft, after all. Other places do seem so cramped up and smothery, but a raft don’t. You feel mighty free and easy and comfortable on a raft† (113). Huck likes to be free and easy like the flowing river. It is undeniable that a slow-flowing river and Huck’s life are inter-related. To both of the characters, who meet up via this entity of water, the river symbolizes a place to bond and safety. Water's Representation of Freedom in Twain's The Adventures of Huckleberry Finn :: essays research papers In the novel, The Adventures of Huckleberry Finn, the Mississippi River serves as a prominent setting. Huck, a rapscallion who runs away from his dad by faking his death, and Jim, a runaway slave who previously knew Huck, meet up on Jackson’s Island via the river. To Jim, the river is a symbol of freedom and a way to learn. To Huck, the river is a symbol of his life and everything he wants. The open waters bring about bonding, fun times, and a safe house for both characters. Amidst the water brings a deeper meaning of the river than just water, it is a great entity that shows freedom. Jim, the runaway slave, uses this river to escape from Miss Watson, his owner, because she planned on selling him for $800. The river flows to the free states, and this is where Jim plans on going with or without Huck. As he is with Huck more and more, Jim learns emotions, like love and compassion, on the river. Jim’s newly shown emotions come into play when the narrator states, â€Å"It was Jim’s voice – nothing ever sounded so good before. I run along the bank a piece and got aboard, and Jim he grabbed me and hugged me, he was so glad to see me† (Twain 112). Love and compassion is shown here by Jim, to Huck. Only freedom has given this runaway slave a way to learn and live like a human being should. Huck, the rapscallion who fakes his death, also has a deep relationship with the river. He relates the river to his life more or less. Since he is a free man, freedom wouldn’t be the right word, more blithe you could say. â€Å"It was kind of lazy and jolly, laying off comfortable all day, smoking and fishing, and no books nor study† (24). Huck’s nature of a care-free person is shown here. He does not like to be cramped up or reformed to anybody he is not. â€Å"[I] said there warn’t no home like a raft, after all. Other places do seem so cramped up and smothery, but a raft don’t. You feel mighty free and easy and comfortable on a raft† (113). Huck likes to be free and easy like the flowing river. It is undeniable that a slow-flowing river and Huck’s life are inter-related. To both of the characters, who meet up via this entity of water, the river symbolizes a place to bond and safety.